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Vaccination

After several experimental vaccines had been described [Jarrett et al., 1975; Jarrett et al., 1974; Pedersen et al., 1979], the first FeLV vaccine in the field was introduced in the USA in1984. This vaccine was based on conventionally prepared FeLV antigens, and it protected cats from FeLV viraemia [Lewis et al., 1981].
A number of FeLV vaccines are now available in Europe. Some of these are based on new developments in recombinant DNA technology. One such vaccine consists of the viral envelope glycoprotein as well as part of the transmembrane protein expressed in E. coli [Kensil et al., 1991], and this was the first genetically engineered small animal vaccine. The most recent FeLV vaccine uses a canarypox virus vector that carries the genes for the envelope glycoprotein and the capsid protein [Tartaglia et al., 1993]. There is a single round of replication by the vector virus following vaccination, resulting in the expression of the inserted FeLV genes. In contrast to other cat vaccines, neutralising antibodies do not develop following vaccination with this product. The protective effect is achieved by stimulating cellular immunity which leads to rapid development of neutralising antibodies if vaccinated cats encounter field virus [Hofmann-Lehmann et al., 2006; Lehmann et al, 1991].
The differences between the various brands of FeLV vaccines are considered to be more significant than those for other feline infectious diseases, and there is evidence that this is reflected in differences in performance, particularly related to efficacy of protection. Comparison of the results of vaccine efficacy studies can be misleading because of differences in the protocols used – such as the route of challenge, the challenge strain used and the criteria for defining protection [Sparkes, 2003]. Different studies on the same vaccine have sometimes led to contrasting results. The first FeLV vaccine and some other vaccines, which have now been withdrawn from the market, have performed very badly in some independent vaccine efficacy studies indicating poor protection.
The European Pharmacopoeia defines certain criteria for assessing the efficacy of protection achieved by FeLV vaccines. This takes into account the difficulty in infecting some healthy control cats with a single experimental challenge and the criteria include a minimum acceptable infectivity rate in controls to confirm that an acceptably strong challenge has been provided. The “natural resistance” of some of the control cats is taken into account in calculating the level of protection achieved by vaccination and this is expressed as the preventable fraction [Scarlett & Pollock, 1991].
Some protocols for studies for assessing vaccine efficacy have been developed based on a “natural” challenge of FeLV – by co-mingling viraemic “challenge” cats with trial cats. Although these protocols are not in agreement with the European Pharmacopoeia, they take account of the natural mode of transmission of FeLV which is generally based not on a singlelarge exposure but chronic exposure over a period of time, usually through cohabiting of infected viraemic cats with susceptible cats, and clinical experts regard this as providing a more realistic indication of the efficacy of protection vaccines are likely to provide in the field.
No FeLV vaccine is likely to provide 100 % efficacy of protection and none prevent infection [Hofmann-Lehmann et al., 2007]. Recent studies have demonstrated that cats that are able to overcome p27 antigenaemia without exception become provirus positive in the blood and also positive for viral RNA in plasma, although at very low levels compared with persistently viraemic cats [Hofmann-Lehmann et al., 2007]. These experiments confirm that FeLV vaccination neither induces sterilising immunity nor protects from infection.
Long term observation of vaccinated cats following experimental challenge indicates that low level RNA viraemia and persistence of low levels of proviral DNA can be considered as not clinically significant and these cats can be regarded as “protected”.
In most circumstances FeLV should be included in the routine vaccination programme for pet cats. It provides good protection against a potentially life-threatening infection and the benefit for most cats considerably outweigh any small risk of serious adverse effects. In situations where the possibility of future exposure to FeLV can be discounted, vaccination is not required. Geographical variations of the prevalence of FeLV may influence the decision whether or not to vaccinate against FeLV. In some European countries FeLV has largely been eradicated and there may be important local variations in the prevalence of FeLV within countries where the virus is still a significant health issue that may be taken into account. The circumstances of individual cats may also be a factor and if it can be assured that a cat will not be exposed to FeLV, vaccination is unnecessary.
However, owners’ circumstances may change which may influence their cats’ lifestyle and lead to potential exposure in a cat that was previously at no risk of encountering FeLV, particularly when moving house. This possibility should be considered especially in kittens presented for primary vaccination.

Primary vaccination

Vaccination should be carried out in all cats that have a potential risk of exposure. In such cases it is recommended that kittens be vaccinated at the age of 8 or 9 weeks and 12 weeks together with core vaccinations. [Brunner et al., 2006]. As the combination of differentimmunogens within one syringe is only legal when the company has registered it for the country of interest; the local veterinary regulations should be carefully consulted.
If the FeLV status is unknown, any cat should be tested for presence of FeLV antigenaemia prior to vaccination in order to avoid "vaccine failures", which are likely when cats infected prior to vaccination develop FeLV-related clinical signs. If FeLV infection prior to vaccination is unlikely, testing may not be needed (e.g. kittens from a FeLV negative mother and father which had no contact with other cats).

Booster vaccinations

No data have been published to support a duration of immunity (DOI) longer than 1 year after primo-vaccination. Therefore, most vaccine producers recommend annual boosters. However, considering the significant lower susceptibility of adult cats to FeLV infection, the ABCD suggests that in cats older than 3-4 years, a booster every two to three years would be sufficient.